The seeds and membranes in chile peppers contain most of the capsaicin, the compound that lends them their mouth-searing qualities. Use caution when handling these chiles. Serranos are hot enough to easily irritate the skin on the hands and it can be painful if their juice comes in contact with the eyes. Wear thin disposable surgical gloves while working with hot chiles, and don’t touch your face until the gloves are removed.
Serrano peppers are often used to add considerable heat to salsas and sauces and can be used with or without their seeds. While they do not need to be peeled, these peppers should be roasted before adding to sauces.